Turning Biological Complexity
into Precision Decisions
Human-Relevant Models. Actionable Insights. Better Translation
Pythia combines its MPS platform, built on proprietary TME-Chip technology, with deep expertise in oncology drug development, translational research, biomarker strategy, and multi-omics integration. We partner with biopharmaceutical companies, academic medical centers, research laboratories, and clinical investigators to generate biologically relevant data and decision-ready insights that support programs from discovery toward the clinic.
Pythia MPS / Microphysiological system
Bring controlled perfusion
to 3D tumor research
The Pythia MPS combines the TME-Chip, Perfusion Hub and Perfusion Controller for parallel
3D tumor-microenvironment culture in translational and preclinical labs.
A CONNECTED SYSTEM
Three components. One experimental setup.
Build your model in the chip. Perfuse it on the Hub. Manage your protocol from the Controller.
TME-Chip
Three culture chambers, one perfusion channel and a micropillar interface with no membrane in the transport path.
Perfusion Hub
Six independent flow paths driven by one peristaltic rotor. Runs inside a standard incubator; chip holders can be customized.
Perfusion Controller
Touchscreen protocol builder for two pumps, with three-point calibration, cycle automation and live run monitoring.
CONSUMABLE
Pythia TME-Chip
The Pythia Tumor Microenvironment-Chip (TME-Chip) is a microfluidic organ-on-a-chip that recreates the human tumor microenvironment in 3D. Three culture chambers, linked by a single perfusion channel, support cell-laden extracellular matrix (ECM) culture of tumor, stromal, and immune cells for drug-response testing, live-cell imaging, and serial effluent sampling.
Key capabilities
3D tumor culture
Each chamber holds a cell-laden ECM; three chambers per chip give replicate cultures under the same perfusion stream.
Multicellular co-culture
Combine tumor cells with fibroblasts, endothelial and immune cells to model tumor–stroma and tumor–immune interactions.
Micro-pillar barrier
A micropillar array with 40 µm gaps separates each chamber from the perfusion channel.
Optical access
Glass bottom in 1 mm or 0.17 mm for brightfield, fluorescence and confocal imaging.
Sample recovery
Collect effluent and retrieve cells for biochemical, secretome and molecular analyses.
INSTRUMENT
Pythia Perfusion Hub
The Pythia Perfusion Hub is a compact perfusion platform with six independent flow paths, built for parallel organ-on-a-chip culture without dedicated infrastructure. A single peristaltic pump drives six physically separated fluid streams in a closed loop, and the whole unit runs inside a standard CO₂ incubator. Standard chip holders fit the Pythia TME-Chip; chip holders and platform size can be customized for other microfluidic devices.
Key capabilities
Six independent flow paths
Each chip runs on a physically separated fluid path with no shared manifold, minimizing cross-contamination.
Single peristaltic pumping
One peristaltic pump drives all six tubes, so every chip is perfused under the same flow conditions.
Easy setup
Reservoirs and tubing sit in fixed positions and chips drop into their holders, with no manifold to assemble.
Customizable format
Standard holders fit the TME-Chip; holders and platform size can be made to fit other organ-on-a-chip devices.
Non-contact fluid handling
Medium stays inside the tubing and never touches the pump, reducing the contamination risk.
Designed for the incubator
Two Hubs fit side by side on one shelf, giving 12 independently perfused chips without a dedicated enclosure.
INSTRUMENT
Pythia Perfusion Controller
The Pythia Perfusion Controller is a programmable touchscreen controller that turns user-defined flow profiles into precise commands for the peristaltic pump of the Pythia Perfusion Hub. Protocol building, live run monitoring, calibration, and program memory are combined in one interface for repeatable microfluidic experiments.
Key capabilities
Protocol builder
Build sequences of clockwise, counterclockwise and static segments, each with a defined flow rate and duration.
Independent dual-hub programming
Run two hubs at the same
time, each with its own flow profile.
Live run monitoring
Rate, direction, accumulated volume, progress and cycle count are shown throughout the run.
Three-point calibration
A guided low-, medium- and high-speed calibration builds a correction curve for your tubing and fluid.
Cycle automation
Repeat selected protocol blocks for a defined number of cycles or continuously.
Safety-oriented control
Run, pause and stop controls with
input-range validation.
FAQ
Does the Controller contain a pump?
No. Each Perfusion Hub includes a peristaltic pump. The Controller provides the touchscreen interface to program and monitor one or two connected Hubs.
Can I use other chip formats?
Yes. Any chip format with one inlet and one outlet is compatible. Our team can discuss the holder and tubing arrangement for your chip.
What is included with the Hub?
A peristaltic pump and chip holders. The Controller, chips, medium reservoirs, and tubing are ordered separately.
Can two Hubs run different protocols?
Yes. One Controller can manage two Hubs, each with its own perfusion program.
Contact Us
Connect with Pythia Biotech to learn more about our products and services,
explore partnering opportunities, or discuss potential collaborations.
